2018 旭硝子財団 助成研究発表会 要旨集

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3 D-aTNASeesaw gate DNAD-aTNA InputDNAInputD-aTNA D-aTNAInputD-DNADNA DNA seesaw gateDNADNAD-aTNAD-DNAD-aTNADNARNA SNAD-RNA→D-aTNA D-aTNARNARNAD-aTNASNARNAD-aTNARNAtoehold exchangeD-aTNA4a4bmiR21(RNA)RNAD-aTNA seesaw gateSNARNASNA5) 2.3. HCRmiR21 Seesaw GateHybridization Chain Reaction (HCR)5amiR21D-aTNA5b 3. HCRSNAmiR21 4. 1) H. Asanuma et al., J. Am. Chem. Soc., 2010, 132, 14702. 2) H. Kashida et al., Angew. Chem. Int. Ed., 2011, 50, 1285. 3) K. Murayama et al., Chem. Commun., 2015, 51, 6500. 4) E. Winfree et al., J. Am. Chem. Soc. 2009, 131, 17303. 5) K. Murayama et al., ChemistrySelect, 2017, 2(20), 5624. 6) N.A. Pierce et al., PNAS, 2004, 101, 15275. 5. 464-8603 Tel: 052-789-2488, Fax: 052-789-2528 E-mail: asanuma@chembio.nagoya-u.ac.jp 4 RNA(miR21)SNAD-aTNA seesaw gate (a) SNA (b) RNA(miR21) 5 (a) HCR(b) miR21 －93－