2017 旭硝子財団 助成研究発表会 要旨集

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GPCR RNAi(RNAi feeding library was constructed by Julie Ahringers group : Source BioScience LifeSciences)3G(GPCR) strsrhsrdsrisrxGPCRRNAi202800GPCRRNAi30eri-1;lin-15BRNAeri-1;lin-15B183akt-1RNAiakt-11akt-10.7GPCR 107akt-1eri-1;lin-15BGPCRRNAi60GPCRGPCR40RNAieri-1;lin15BASJGPCR 3. GPCR40800GPCRGPCRGPCRRNAiGPCRGFPASJ 4. Caterina, M.J., Rosen, T.A., Tominaga, M., Brake, A.J., and Julius, D. (1999). A capsaicin-receptor homologue with a high threshold for noxious heat. Nature 398, 436-441. Komatsu, H., Mori, I., Rhee, J.S., Akaike, N., and Ohshima, Y. (1996). Mutations in a cyclic nucleotide-gated channel lead to abnormal thermosensation and chemosensation in C. elegans. Neuron 17, 707-718. Kuhara, A., Okumura, M., Kimata, T., Tanizawa, Y., Takano, R., Kimura, K.D., Inada, H., Matsumoto, K., and Mori, I. (2008). Temperature sensing by an olfactory neuron in a circuit controlling behavior of C. elegans. Science 320, 803-807. Liu, J., Ward, A., Gao, J., Dong, Y., Nishio, N., Inada, H., Kang, L., Yu, Y., Ma, D., Xu, T., et al. (2010). C. elegans phototransduction requires a G protein-dependent cGMP pathway and a taste receptor homolog. Nat Neurosci 13, 715-722. Ohta, A., Ujisawa, T., Sonoda, S., and Kuhara, A. (2014). Light and pheromone-sensing neurons regulates cold habituation through insulin signalling in Caenorhabditis elegans. Nature communications 5, 4412. Ujisawa, T., Ohta, A., and Kuhara, A. (2014a). Long-term calcium imaging of ASJ sensoryneuron controlling cold tolerance in C. elegans. Protocol Exchange. Ujisawa, T., Ohta, A., M., O., S., S., and Kuhara, A. (2014b). Cold tolerance assay for studying cultivation-temperature-dependent cold habituation in C. elegans. Protocol Exchange. 5. 658-8501 -- 078-431-4341 E-mailkuhara@center.konan-u.ac.jp −23−